Roseus hairy roots

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Clinically important anti-cancer drugs vinblastine and vincristine are solely synthesized by the terpenoid indole alkaloid TIA pathway in Catharanthus roseus. The transgenic C. Although genetic modification is targeted to one gene in the TIA pathway, it could trigger global transcriptional changes that can directly or indirectly affect TIA biosynthesis.

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Shanks, Ka Yiu San. Stably transformed transgenic hairy root cultures have the potential to be a valuable production platform for a variety of secondary metabolites. This study reports that a transgenic hairy root culture of Catharanthus roseus has been stably maintained for over 4.

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The overall goals are to determine the metabolic effects of genetic manipulations of some key enzymes in the indole and non-mevalonate terpenoid pathways on flux through the indole alkaloid synthesis pathways, with special emphasis on the interplay between primary and secondary metabolism on the final alkaloid productivity. The specific objectives are to: 1 construct and characterize transgenic hairy root lines overexpressing key enzymes, such as ORCA3, G10H, DXS, DXR and AS-beta under the control of a recently characterized inducible promoter system; 2 develop methods for NMR- based metabolic flux maps; 3 perform metabolic characterization of these first generation transgenic lines; 4 develop techniques for co-transformation that allow the introduction of multiple genes; 5 construct and characterize a second generation of transgenic lines expressing strategic combinations of genes as determined from the results of the first generation transgenic lines above. Rice University".

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CiteScore 0. The induction of hairy roots in Arnica montana L. The frequency of genetic transformation varied from 4.

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Among the pharmacologically important terpenoid indole alkaloids produced by Catharanthus roseus are the anti-cancer drugs vinblastine and vincristine. These two drugs are produced in small yields within the plant, which makes them expensive to produce commercially. Metabolic engineering has focused on increasing flux through this pathway by various means such as elicitation, precursor feeding, and introduction of genes encoding specific metabolic enzymes into the plant.

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JavaScript is disabled for your browser. Some features of this site may not work without it. Metabolic engineering of the terpenoid indole alkaloid pathway of Catharanthus roseus hairy roots.

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Sang Jun SimH. Chang, J. Liu, K.

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Catharanthus roseus has traditionally been cultivated in China for use in herbal medicine. However, the biologically beneficial agents in this plant have not previously been able to be isolated for mass production. This invention isolates eleven natural products from cultures of C.

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